POSTERS
AACR 2026 | poster #6201
PaintScape enables multiomic in situ, direct visualization of spatial 3D genome architecture of single cells in intact fresh frozen Glioblastoma tissue in native tumor microenvironments
highlights
PaintScape enabled identification, characterization, and direct in situ visualization of single cell spatial 3D genome structure of various tissue microenvironments in 30-µm fresh frozen (FF) glioblastoma (GBM) tissue.
The multiomic jebFISH protocols for tissue samples enabled identification of specific cell types or tissue regions for further direct visualization and analysis of 3D genome organization..
The power of the single-cell nature of the data allows for unprecedented filtering, analysis, and comparison of genomic landscapes or specific chromosomal aberrations in specific single cells as well as specific subpopulations of cells.
Distinct structural genotypes and genome conformations for individual single cells and cell subpopulations were revealed by jebFISH on the PaintScape System. (e.g., Chr10 loss)
PaintScape characterized and revealed distinct structural genotypes and genome conformation differences in hypoxic vs normoxic tissue regions.
CNVs in GBM and hypoxia related genes and 3D genome differences (Chr10 further from other chromosomes in hypoxic)
Chr7 3N cells: CNVs in GBM and hypoxia related genes and 3D genome differences (Chr7 and Chr10 differential interactions)
PaintScape revealed high levels of MET amplification forming ecDNA with neighboring genes (CAPZA2 and ST7) in hypoxic and normoxic regions