In essence, jebFISH makes it possible to directly see chromosomal structure—territories, conformation, and aberrations (including copy number variation, segmented duplications, translocations, and ecDNA formation)—by enabling highly multiplexed “painting” of genomic regions of interest and visualizing chromosome traces.
jebFISH Technology thus reveals insights into how the location, conformation, and copy number of genomic regions of interest (in the context of their chromosomal and genome architecture) correlate with and affect cell programming and disease.
jebfish-enabled biological insights
jebFISH Technology encompasses innovative, optimized protocols that leverage both novel, proprietary advancements to fluorescence in situ hybridization (FISH) as well as specially designed readout probes with sophisticated propietary barcoding. That combination, together with robust, high-performance PaintScape imaging instrumentation, powers jebFISH with the breakthrough ability to paint regions of the genome simultaneously at extremely high multiplex and achieve efficient detection and readout within the constrained space of a tiny cell nucleus (often measuring 5–10µm). Hundreds of genomic loci targets can be simultaneously painted.
The innovations of jebFISH genome painting enable unprecedented, highly multiplexed, and high-resolution direct visualization of chromosome architecture in cell nuclei and open the way for novel insights into cell programming in human health and disease.
EXAMPLES of jebFISH 3D Genome Insights
in MCF-7 cells
